How do I convert pIC50 to IC50?

How do I convert pIC50 to IC50?

Simply stated, pIC50 is the negative log of the IC50 value when converted to molar. For example, an IC50 of 1 uM is 1 x10^-6 M and that’s equal to a pIC50 of 6. An IC50 of a nanomolar compound is 1 x10^-9 M, which is a pIC50 of 9.

How is IC50 value calculated?

The simplest estimate of IC50 is to plot x-y and fit the data with a straight line (linear regression). IC50 value is then estimated using the fitted line, i.e., Y = a * X + b, IC50 = (0.5 – b)/a.

How do you convert pIC50 to nM?

An IC50 of 1 nM is 10-9 M, which is pIC50 = 9.0. An IC50 of 10 nM is 10-8 M, which is pIC50 = 8.0.

How do you find the IC50 of a graph?

To calculate the IC50 value, from the equation of the graph that you got (Y = MX-C), change Y to 50 (50= MX-C) then make X the subject of the formula. The X value should be your IC50 value.

What is a good IC50 for a drug?

In most cases, the IC50 of the best candidate compound should be lower to 10 micromolar, acceptable for NIH to screen the NCI60 program. In addition, the drug design should think over the related problem about the absorption, distribution, metabolism and excretion in our body.

Is higher IC50 better?

The IC50 value is correlated with drug potency, i.e. the amount of drug necessary to produce the effect—the lower the IC50 value the more potent the drug [33].

What is the Cheng Prusoff equation?

The Cheng-Prusoff equation defines the theoretical relation- ship between the measured IC50 for a competitive inhibitor of given Ki, the concentration of labeled ligand, and the KD of the ligand-receptor interaction.

What is IC90?

The EC10 (for example) is defined as the concentration that gives you a response 10% of the way between the Bottom and Top plateaus. In situations where the response goes down as you increase dose, you may think of this as the “IC90” since it gives 90% of the maximum inhibition.

How do you find the LC50 of a graph?

Step 4: Find the LC50 Method A: Using your hand drawn graph, either created by eye or by calculating the regression by hand, find the probit of 5 in the y-axis, then move down to the x-axis and find the log of the concentration associated with it. Then take the inverse of the log and voila! You have the LC50.

Should IC50 be low or high?

Is a low IC50 better?

Therefore, the lower dose the IC50 of candidate was, the higher potentially it become the new drug for any diseases. If the best known candidate has an IC50 of 100 micromolar, and your compounds have an IC50 below 100 micromolar, yours is better than the known compounds and should “theoretically” be publishable.

What is a good IC50 number?