How much does a fluorescence microscope cost?

A fluorescence microscope can cost between $2,400 and $21,000+ depending on the specifications and customizations that you require.

Is fluorescence microscope expensive?

For those of you who haven’t visited the websites of Carl Zeiss or Nikon lately, fluorescent microscopes are expensive. Typically, microscopes of the sort required by pathologists to make diagnosis are around $2,000, weigh something on the order of a small child, and require mains power.

In what circumstances would you use an upright an inverted and a fluorescence microscope?

In cell biology, upright microscopes are used for phase contrast or widefield fluorescence microscopy of living cells or samples that are squeezed between a slide and coverslip. An additional application is the microscopy of fixed cells or tissue sections.

What are some disadvantages of fluorescence microscopy?

The disadvantage of fluorescent microscopy is that the addition of probes and dyes to a membrane system can potentially interfere with the properties of the liposomal delivery system (Bouvrais et al., 2010; Bibi et al., 2011; Murphy and Davidson, 2012b).

How do you focus a fluorescence microscope?

Peek through the eyepiece and slowly make the necessary adjustments to bring your sample into focus. The coarse knobs are there to lift or lower the stage while the fine knobs are there to provide a sharper and clearer image of your specimen.

What is the resolution of a fluorescent microscope?

Spatio-temporal visualization of cellular structures by fluorescence microscopy has become indispensable in biology. However, the resolution of conventional fluorescence microscopy is limited by diffraction to about 180 nm in the focal plane and to about 500 nm along the optic axis.

What are the advantages of fluorescence microscope?

Fluorescence microscopy is one of the most widely used tools in biological research. This is due to its high sensitivity, specificity (ability to specifically label molecules and structures of interest), and simplicity (compared to other microscopic techniques), and it can be applied to living cells and organisms.

What is YFP HYQ yellow fluorescent protein filter combination?

The YFP HYQ yellow fluorescent protein filter combination is designed to provide optimal excitation for enhanced yellow variants of GFP.

What is YFP and emgfp used for?

These proteins are commonly used with the YFP and EmGFP for multi-color applications. Configured for bacterial expression and fluorescent protein purification, these vectors can also be used for subcloning to construct customized mammalian expression vectors.

How can I detect emgfp and YFP fusion proteins using FITC?

Expression of the Invitrogen Vivid Colors EmGFP and YFP fusion proteins can be detected by fluorescence microscopy or flow cytometry using standard FITC filter sets. For optimal visibility, it is recommended that you use a filter set optimized for detection within the excitation and emission ranges for each fluorescent protein (see table below).

What is the Nikon yellow fluorescent protein fluorescence filter category?

The Nikon yellow fluorescent protein fluorescence filter category comprises a single high-performance balanced combination, which effectively extends the fluorescent protein detection capabilities afforded by the three green fluorescent protein (GFP) filter sets to the longer wavelength enhanced yellow chromatic variants of GFP (YFP and EYFP).