What does MMP 7 do?

MMP-7 plays a role in remodeling of tissues involved in development and reproduction such as the uterus, and could play a role in remodeling following tissue injury. MMP-7 degrades ECM components and cleaves cell-surface molecules such as Fas–ligand, pro-TNF-α, syndecan-1, and E-cadherin to generate soluble forms.

What is MMP release?

MMPs participate in cleavage and domain release of cell surface and membrane-spanning proteins. Shedding is performed by membrane-type MMPs, MMPs that are bound to membrane receptors (such as a subpopulation of MMP-2 and MMP-9) [43] and soluble MMPs.

What produces MMP?

MMPs are produced by many cell types, including lymphocytes and granulocytes, but in particular by activated macrophages (17). Their generation of chemotactic fragments from ECM proteins may also contribute to the recruitment of inflammatory cells (22, 40).

How many MMP are there?

In vertebrates, there are 28 different types of MMPs [1,2,8,9,11,12,13,16,17], at least 23 are expressed in human tissue [1].

Are MMPs Collagenases?

Background: Members of the family of matrix metalloproteinases (MMPs; also called collagenases or matrixins) are key enzymes in matrix degradation. They function at neutral pH and can digest synergistically all the matrix macromolecules.

Is MMP-9 a cytokine?

The effect of cytokines on MMP-9 production in human macrophages was verified by previous incubation of cytokines on these cells in culture, and analyzed by zymography. cruzi Y or Colombian strains produced increased levels of MMP-9, which was related to the production of cytokines such as IL-1β, TNF-α and IL-6.

Is MMP7 secreted or secreted?

Since MMP7 is a secreted protein [6, 38, 39], cells were grown in the absence of serum and the supernatant collected for the indicated cell types. The proteins in the supernatant were precipitated using acetone as described in the Materials and Methods and a Western blot performed for MMP7.

Is MMP7 required for in-vitro cell migration and invasion and tumor formation?

Using vector driven RNA interference, it was demonstrated that MMP7 was required for in-vitro cell migration and invasion and tumor formation in vivo. The increase in MMP7 levels was due to the increase in levels of the Phosphatase of Regenerating Liver3 (PRL3), which is observed upon PKP3 loss.

What is the role of MMp7 in fibrosis?

MMP7 was initially identified in an unbiased microarray screen of patients with IPF. MMP7-null mice were then found to be protected from fibrosis in the bleomycin model.

Does MMP7 over-expression increase the risk of PKP3 loss?

The results suggest that MMP7 over-expression may be one of the mechanisms by which PKP3 loss leads to increased cell invasion and tumor formation. Introduction