What is a immunochromatographic assay?

The immunochromatographic assay is a simple and rapid test which represents a suitable alternative to the conventional subculture method for the primary identification of Mycobacterium tuberculosis complex in liquid cultures of BacT/Alert automated system.

How do immunochromatographic tests work?

Immunochromatography (or lateral flow tests) combines separation of the sample molecules and reagents based on migration on a solid support by capillary flow. The identification and detection procedures are based on the antigen–antibody immune reaction.

What is sandwich immunoassay?

Sandwich Immunoassay (ELISA) A sandwich immunoassay is a method using two antibodies, which bind to different sites on the antigen or ligand (Figure 1). The capture antibody, which is highly specific for the antigen, is attached to a solid surface.

What is involved in the reaction or in the immunochromatographic assay?

Immunochromatographic tests rely principally on the capture of the target antigen (or sometimes antibodies) from various specimens. The assay utilizes antibodies mounted on a paper strip or a nitrocellulose membrane as the immobile capture antibody (test area).

What is immunochromatographic strip?

The immunochromatographic strip (ICS) test, or lateral flow assay, is an antibody-based technique, referring to the migration of antigen-antibody complexes through a filter matrix such as nitrocellulose strips [21,22].

How do immunoassays work?

Immunoassay tests work by using antibodies to detect small biological substances in the blood and other bodily fluids. The method follows the aspect that particular antigens binds to specific newly introduced antibodies, thereby stimulating an immune response.

How do lateral flow assays work?

LFDs use immunoassay technology using nitrocellulose membrane, coloured nanoparticles (or labels), and typically antibodies, to produce results. When a sample is added, the sample will flow along the test device passing through the conjugate pad into the nitrocellulose membrane and then onto the absorbent pad.

What is sandwich ELISA?

The sandwich ELISA is a type of Enzyme-linked immunosorbent Assay that uses two antibodies: a capture antibody and a detection antibody. The purpose of any ELISA is to detect the presence of a target antigen in a sample.

What are ELISA assays?

ELISA (enzyme-linked immunosorbent assay) is a plate-based assay technique designed for detecting and quantifying soluble substances such as peptides, proteins, antibodies, and hormones. Other names, such as enzyme immunoassay (EIA), are also used to describe the same technology.

What is the application of Immunochromatography?

Immunochromatographic methods are widely used in animal quarantine for the detection of antigens or antibodies in diseases such as swine fever, porcine reproductive and respiratory syndrome (PRRS), parvovirus infection, infectious bursal disease, Newcastle disease, rabies, and diseases caused by other infectious …