What is in a Leukopak?

Leukopaks typically contain up to 50% T cells, 20% monocytes, 10% B cells, and 10% NK cells, 3% granulocytes, and 3 % hematocrit. Actual percentages will vary from donor to donor.

What is a Leukopak used for?

Erythrocytapheresis refers to the removal of red blood cells, and can be used as a Category I treatment for sickle cell anemia. Sickled cells may be sorted and replaced with normal RBCs from a donor (Swerdlow, 2006).

How much does a Leukopak cost?

Leukopak

How many PBMCs are in a Leukopak?

How many cells (PBMCs) can I isolate from a Leukopak? Usually around 8×109 PBMCs from a Fresh Leukopak.

How does leukapheresis machine work?

Apheresis units incorporate polyvinyl tubing that draws blood from the patient and moves it through centrifuges and/or filters to separate blood products. The blood is then returned to the patient via tubing or is collected in bags, often suspended from a pole, for donation or disposal.

What’s the difference between leukapheresis and apheresis?

Apheresis is a procedure used to fractionate whole blood into its individual components. Leukapheresis is one type of apheresis where leukocytes (white blood cells) are selectively removed. This procedure is commonly used for blood transfusions to remove donor leukocytes from being transferred to the recipient.

How do you process Leukopak?

Aliquot the diluted leukopak contents into the appropriate tubes for centrifugation. We recommend using 50 mL conical tubes for centrifugation. Centrifuge the cells at 300 x g for 10 minutes at room temperature (15 – 25ºC) with the brake ON. Carefully remove the supernatant without disturbing the cell pellet.

Are neutrophils in buffy coat?

The buffy coat is usually whitish in color, but is sometimes green if the blood sample contains large amounts of neutrophils, which are high in green-colored myeloperoxidase. The layer beneath the buffy coat contains granulocytes and red blood cells.

What is leukapheresis?

Leukapheresis is a medical procedure in which white blood cells (leukocytes) involved in the body’s immune response are separated from a sample of blood. It is a specific form of apheresis in which a component of blood, such as red blood cells or platelets, are extracted while the remaining blood is returned to circulation.

What are the treatments for leukapheresis?

Antibiotics or antifungal drugs may be used to treat an infection or to prevent infection in those with a weakened immune system. Leukapheresis is a scheduled procedure that uses a specialized apheresis machine to remove blood under gentle pressure, spin it to remove the desired cells, and return the blood to the body at a stable temperature.

What is thertherapeutic leukocytapheresis?

Therapeutic leukocytapheresis (or leukapheresis) is a procedure in which white blood cells (WBCs) are selectively removed from the patient’s circulation. This procedure is most commonly performed for hyperleukocytosis, which is defined as a circulating WBC count >100,000/μL, typically occurring in the setting of leukemia.

What is a leukopak?

Leukopaks are highly concentrated, low-volume apheresis collections from normal donors, with Institutional Review Board (IRB)-approved consent forms and protocols. Each full-sized leukopak is produced from ~2 – 3x blood volumes using the Spectra Optia® Apheresis System, with acid-citrate-dextrose solution A (ACDA) as the anticoagulant.