What is the source of PstI?

Product Source A E. coli strain that carries the PstI gene from Providencia stuartii 164 (ATCC 49762).

Does PstI have star activity?

Engineered for improved performance. 100% activity in rCutSmart Buffer. Time-Saver™ qualified for digestion in 5-15 minutes. Reduced star activity.

What is SAL1?

The nucleotidase/phosphatase SAL1 is a negative regulator of drought tolerance in Arabidopsis. Plant J. 1365-313X.

Is pvu 1 a restriction site?

PvuI is a restriction endonuclease that is used for molecular biological applications to cleave DNA at the recognition sequence 5′-CGAT/CG-3′ to generate fragments with 3′-cohesive termini.

What does PstI stand for?

PSTI

What type of enzyme is PstI?

PstI is a type II restriction endonuclease isolated from the Gram negative species, Providencia stuartii.

Why is star activity bad?

Star activity, also known as off-target cleavage, is an undesirable but intrinsic attribute of restriction enzymes. It is the loss of enzyme specificity resulting in cleavage at sites that are similar, but not identical to, the canonical recognition site for an enzyme. High enzyme:DNA ratio, or overdigestion.

What type of nucleic acid does the restriction endonuclease BamHI cut?

Restriction endonuclease BamHI bound to a non-specific DNA. BamHI (pronounced “Bam H one”) (from Bacillus amyloliquefaciens) is a type II restriction endonuclease, having the capacity for recognizing short sequences (6 bp) of DNA and specifically cleaving them at a target site.

How many restriction sites are there in pBR322?

It contains the origin of replication of pMB1, and the rop gene, which encodes a restrictor of plasmid copy number. The plasmid has unique restriction sites for more than forty restriction enzymes. Eleven of these forty sites lie within the TetR gene.

What is the role of rop in pBR322?

The ‘rop’ gene which is present in the pBR322 codes for the rop protein. Its main function is in decreasing the copy number and so this gene is involved in the replication of the plasmid. -When a plasmid is used to make copies of a foreign DNA sequence, it is called a cloning vector, or simply a vector.

How many fragments are produced by PstI?

c) For those two restriction endonucleases, each DNA fragment in the single digest is cut by PstI into two DNA fragments in the double digest (i.e. the restriction endonuclease plus PstI).

What are the number of PstI sites?

Unit Definition: One unit is the amount of enzyme required to completely digest 1 μg of Lambda DNA (28 sites) in 1 hour in a total reaction volume of 50 μl. Enzyme activity was determined in the recommended reaction buffer.