Tips and Tricks

Where is PsiI restriction enzyme isolated?

Where is PsiI restriction enzyme isolated?

PsiI, a novel restriction endonuclease produced by the bacterial strain Pseudomonas sp. SE-G49, has been isolated and characterized. The enzyme cleaves DNA in the middle of its palindromic recognition sequence 5′-TTA downward arrow TAA-3′.

What is SmaI restriction enzyme?

SmaI is a restriction endonuclease used to cut DNA at the recognition sequence 5′-CCC/GGG-3′, generating DNA fragments with blunt termini.

What is pst1 restriction enzyme?

PstI is a restiction enzyme used in molecular biology applications to cleave DNA at the recognition sequence 5′-CTGCA/G-3′, generating DNA fragments with 3′- cohesive termini.

What does PSII do?

PSII is the multisubunit chloroplast membrane-associated pigment–protein complex that uses the energy of sunlight to drive the oxidation of water, evolving oxygen, donating electrons into the photosynthetic electron transfer chain, and depositing protons into the thylakoid lumen.

Does SmaI produce blunt ends?

You can also simply cut the DNA with a restriction endonuclease that leaves blunt ends. SmaI and EcoRV are two such endonucleases that can be used to linearize circular plasmids or cut off sticky ends leaving blunt ends ready for ligation (Fig. 3).

Is SmaI blunt end?

SmaI is an isoschizomer of XmaI. SmaI produces blunt-ended fragments whereas XmaI produces a 5´ extension. SmaI has a half-life of 15 minutes at 37°C.

What is the restriction site for pstl?

Thermo Scientific PstI restriction enzyme recognizes CTGCA^G sites and cuts best at 37°C in O buffer (Isoschizomers: BspMAI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.

What is 2genscript restriction enzyme map analysis tool?

GenScript Restriction Enzyme Map Analysis Tools ** This online tool helps you analyze restriction enzyme cutting maps. Paste in DNA Sequence: Free Sample Promotion Codes & Coupons Quick Order Open 24 Hours

How do you do a restriction map on a DNA sequence?

Restriction Map Generator. This tool analyzes a DNA sequence to identify Restriction Enzyme Sites and generate a comprehensive map overview of their locations within the DNA sequence. Enter a DNA sequence in the box below to analyze the sequence for restriction sites and generate a restriction map.

What is one unit of enzyme activity?

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Not sensitive to CpG, dcm, or dam methylation. Why Choose Recombinant Enzymes? Is PsiI activity sensitive to dam, dcm or mammalian CpG methylation?