What is dsDNA Crithidia luciliae?

Crithidia luciliae is a parasite that uses the housefly as its host. It contains a kinetoplast, a modified mitochondria that contains a dense collection of circularized dsDNA that can be utilized as a substrate alongside patient serum for the detection of antibodies to dsDNA.

What is dsDNA by Crithidia?

Description: dsDNA Crithidia luciliae is an indirect immunofluorescent assay for the screening and semi-quantitative determination of anti-double stranded DNA (dsDNA) in human serum.

What is crithidia Luciliae test?

Fluorescence of the kinetoplast of Crithidia luciliae was employed as a test to detect anti-DNA antibodies in 179 serum samples from 95 patients with various immunological diseases. The test was positive only in patients with systemic lupus erythematosus (SLE).

What are the criteria for diagnosing lupus?

Lupus: Criteria for Diagnosis

• Butterfly (malar) rash on cheeks.
• Rash on face, arms, neck, torso (discoid rash)
• Skin rashes that result from exposure to sunlight or ultraviolet light (photosensitivity)
• Mouth or nasal sores (ulcers), usually painless.
• Joint swelling, stiffness, pain involving two or more joints (arthritis)

What is the Crithidia luciliae if assay?

The Crithidia luciliae IF assay is also widely used. This test detects antibodies that bind to the dsDNA in the kinetoplast of the Crithidia. It is relatively specific for anti-dsDNA antibodies. In particular, because the kinetoplast consists solely of dsDNA, anti-ssDNA antibodies do not bind.

What is the IFA order code for dsDNA Crithidia luciliae?

096346 dsDNA Crithidia luciliae IFA 6457-6 096346 dsDNA Crithidia luciliae IFA 6457-6 Reflex Table for dsDNA Crithidia luciliae IFA Order Code Order Name Result Code Result Name UofM Result LOINC Reflex 1 096347 dsDNA Crithidia luciliae Titer 096347 dsDNA Crithidia luciliae Titer 34187-5 Test Menu Right Side Menu Find a Test New & Updated Tests

What is the refreflex 1 test code for Crithidia luciliae?

Reflex 1 096347 dsDNA Crithidia luciliae Titer 096347 dsDNA Crithidia luciliae Titer 34187-5 Test Menu Right Side Menu Find a Test New & Updated Tests Test Resources Patient Test Information For Providers Please login to order a test Order a Test © 2021 Laboratory Corporation of America® Holdings and Lexi-Comp Inc. All Rights Reserved.

What is anti double stranded DNA (dsDNA) IgG antibody?

Double-Stranded DNA (dsDNA) Antibody, IgG by IFA (using Crithidia luciliae) Positivity for anti-double stranded DNA (anti-dsDNA) IgG antibody is a diagnostic criterion of systemic lupus erythematosus (SLE). The presence of the anti-dsDNA IgG antibody is identified by IFA titer ( Crithidia luciliae indirect fluorescent test [CLIFT]).